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htb 75 detroit 562 atcc  (ATCC)


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    ATCC htb 75 detroit 562 atcc
    Htb 75 Detroit 562 Atcc, supplied by ATCC, used in various techniques. Bioz Stars score: 97/100, based on 1189 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/htb+75+detroit+562+atcc/Caov-3/pm41569853-280-156-159
    Average 97 stars, based on 1189 article reviews
    htb 75 detroit 562 atcc - by Bioz Stars, 2026-09
    97/100 stars

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    Related Articles

    Mutagenesis:

    Article Title: Immune gene diversity and STING1 variants in shaping cancer immunity across different genetic ancestry populations.
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Phospho-TBK1/NAK (Ser172) Cell Signaling Technology Cat #5483; RRID: AB_2737027 TBK1/NAK (D1B4) Cell Signaling Technology Cat #3504; RRID: AB_2255663 Phospho-Stat1 (Tyr701) Cell Signaling Technology Cat #7649; RRID: AB_10950970 Stat1 (D1K9Y) Cell Signaling Technology Cat #14994; RRID: AB_2737027 STING (D2P2F) Cell Signaling Technology Cat #13647; RRID: AB_2732796 β-Actin Cell Signaling Technology Cat #4970; RRID: AB_2223172 Anti-mouse IgG Cell Signaling Technology Cat #7076; RRID: AB_330924 Anti-rabbit IgG Cell Signaling Technology Cat #7074; RRID: AB_2099233 Chemicals, peptides, and recombinant proteins RBN2397 Selleckchem Cat# S8993 2’3’-cGAMP Invivogen Cat# tlrl-nacga23-1 poly(dA:dT) Invivogen Cat# tlrl-patn-1 FuGENE 6 Promega Cat# E2691 Lipofectamine 2000 Invitrogen Cat#11668019 MTT Sigma-Aldrich 475989 Crystal violet solution Sigma-Aldrich HT901 Polybrene infection/transfection reagent Sigma-Aldrich TR-1003-G Puromycin Sigma-Aldrich P8833 Hygromycin Roche 10843555001 Blotting-grade blocker Bio-Rad 1706404 Immobilon forte western HRP substrate Millipore Sigma WBLUF0500 CellTracker TM Blue CMF2HC Dye Invitrogen C12881 Critical commercial assays rhAmp® Genotyping Master Mix IDT Cat#1076016 rhAmp® Reporter Mix w/Reference IDT Cat#1076022 STING1 R71H rs11554776 IDT Cat# CD.GT.KJQS7656.1 STING1 G230A rs78233829 IDT Cat# Hs.GT.rs78233829.G.1 STING1 R232H rs1131769 IDT Cat# CD.GT.HPNL6434.1 STING1 R293Q rs7380824 IDT Cat# Hs.GT.rs7380824.T.1 High-Capacity RNA-to-cDNA Kit Applied Biosystems Cat#4368813 PowerUp SYBR Green Applied Biosystems Cat# A25742 Mammalian protein extraction reagent Pierce Cat# 78505 BCA protein assay kit Pierce Cat# 23225 Transwell Permeable Supports Corning Cat# 3422 Human IFN-beta DuoSet ELISA kit R&D Systems Cat# DY814-05 QUANTI-Blue TM Solution Invivogen Cat# rep-qbs QUANTI-Luc TM 4 Lucia/Gaussia Invivogen Cat# rep-qlc4lg1 Deposited data 1000 Genomes Phase 3 data 1000 Genomes project https://www.internationalgenome.org/ data-portal/data-collection/grch38 SNP array data for TCGA TCGA project https://gdc-portal.nci.nih.gov RNAseq data for TCGA UCSC Toil RNAseq Recompute Compendium https://xenabrowser.net/datapages/ ?hub=https://toil.xenahubs.net:443 Segmentation data for TCGA the GDAC Firehose of the Broad Institute http://gdac.broadinstitute.org/ (Continued on next page) 18 Cell Reports 45, 116882, February 24, 2026 .. REAGENT or RESOURCE SOURCE IDENTIFIER Mutation profiles for TCGA TCGA Multi-Center Mutation Calling in Multiple Cancers (MC3) project https://doi.org/10.7303/syn7214402 Fusion profiles for TCGA TumorFusions data portal http://tumorfusions.org/ Genomic profiles for cancer cell lines the Dependency Map (DepMap) portal https://depmap.org/portal/ CRISPR for cancer cell lines the Score projects https://doi.org/10.6084/m9.figshare.c .5289226.v1 TCGA tumors infiltration TIMER2.0 http://timer.cistrome.org RNA sequencing This paper GEO: GSE314074 Data generated in this study This paper http://52.25.87.215/IDmap/ Experimental models: Cell lines 293T ATCC Cat #CRL-3216; RRID: CVCL_0063 CAL27 ATCC Cat #CRL-2095; RRID: CVCL_1107 NK-92 ATCC Cat #CRL-2407; RRID: CVCL_2142 Jurkat ATCC Cat #TIB-152; RRID: CVCL_0065 HCC1143 ATCC Cat #CRL-2321; RRID: CVCL_1245 NCI-H1373 ATCC Cat #CRL-5866; RRID: CVCL_1465 BHY DSMZ Cat #ACC-404; RRID: CVCL_1086 293-Dual TM Null Cells InvivoGen Cat #293d-null TOV-21G ATCC Cat # CRL-3577; RRID: CVCL_3613 SK-OV-3 NCI Development Therapeutics Program RRID: CVCL_0532 HARA JCRB Cat # JCRB1080.0; RRID: CVCL_2914 SNG-M JCRB Cat # JCRB0179; RRID: CVCL_1707 A-253 ATCC Cat# HTB-41 Caov-3 ATCC Cat# HTB-75 Detroit 562 ATCC Cat# CCL-138 DU 145 ATCC Cat# HTB-81 FaDu ATCC Cat# HTB-43 HCC1937 ATCC Cat# CRL-2336 HCC1954 ATCC Cat# CRL-2338 HCC38 ATCC Cat# CRL-2314 Hs 578T ATCC Cat# HTB-126 HT-29 ATCC Cat# HTB-38 LoVo ATCC Cat# CCL-229 MDA-MB-231 ATCC Cat# HTB-26 MDA-MB-361 ATCC Cat# HTB-27 MES-SA ATCC Cat# CRL-1976 OVCAR-8 NCI Development Therapeutics Program RRID:CVCL_1629 PEO1 Sigma Aldrich Cat# 10032308 SCC-9 ATCC Cat# CRL-1629 SCC-15 ATCC Cat# CRL-1623 SCC-25 ATCC Cat# CRL-1628 SK-BR-3 ATCC Cat# HTB-30 SK-CO-1 ATCC Cat# HTB-39 SW 620 ATCC Cat# CCL-227 U-87 MG ATCC Cat# HTB-14 UPCI-SCC-040 DSMZ Cat# ACC-660 UPCI-SCC-074 DSMZ Cat# ACC-664 (Continued on next page) Cell Reports 45, 116882, February 24, 2026 19 .. REAGENT or RESOURCE SOURCE IDENTIFIER UWB1.289 ATCC Cat# CRL-2945 Oligonucleotides 5’ STING1 gRNA forward: 5 ′ -CACCGAGAATCTAGACAGACGGGCG-3 ′ This paper NA 5’ STING1 gRNA reverse: 5 ′ -AAACCGCCCGTCTGTCTAGATTCTC-3 ′ This paper NA 3’ STING1 gRNA forward: 5 ′ -CACCGTGCTCCACACAGACACACAT-3 ′ This paper NA 3’ STING1 gRNA reverse: 5 ′ -AAACATGTGTGTCTGTGTGGAGCAC-3 ′ This paper NA STING1 knockout allele forward: 5 ′ -TGCCATCCTAGCCTCACTCTCCA-3 ′ This paper NA STING1 knockout allele reverse: 5 ′ -AAGGCCCCCACTGACTCTGTCTT-3 ′ This paper NA STING1 WT allele forward: 5 ′ -ACCTGTGGTCTCCCTGGGTC-3 ′ This paper NA STING1 WT allele reverse: 5 ′ -GGCAGGGCTAGGCATCAAGG-3 ′ This paper NA Forward primer for CXCL10: 5 ′ -CCACGTGTTGAGATCATTGC-3 ′ This paper NA Reverse primer for CXCL10: 5 ′ -CCTCTGTGTGGTCCATCCTT-3 ′ This paper NA Forward primer for CCL5: 5 ′ -TACACCAGTGGCAAGTGCTC-3 ′ This paper NA Reverse primer for CCL5: 5 ′ -TACACCAGTGGCAAGTGCTC-3 ′ This paper NA Forward primer for STAT1: 5 ′ -ATGGCAGTCTGGCGGCTGAATT-3 ′ This paper NA Reverse primer for STAT1: 5 ′ -CCAAACCAGGCTGGCACAATTG-3 ′ This paper NA Forward primer for IFNB1: 5 ′ -TGCTCTGGCACAACAGGTAG-3 ′ This paper NA Reverse primer for IFNB1: 5 ′ -CAGGAGAGCAATTTGGAGGA-3 ′ This paper NA Forward primer for GAPDH: 5 ′ -ACACCATGGGGAAGGTGAAG-3 ′ This paper NA Reverse primer for GAPDH: 5 ′ -AAGGGGTCATTGATGGCAAC-3 ′ This paper NA Recombinant DNA LentiCRISPRv2 Sanjana et al. 83 Addgene #52961 pMSCV-hygro-STING Cerboni et al. 84 Addgene #102598 pMSCV-hygro-STING HAQ Cerboni et al. 84 Addgene #102600 pSBbi-RP Hu CXCR3 Unpublished Addgene #183255 Software and algorithms SnpEff Cingolani et al. 85 https://pcingola.github.io/SnpEff/ popgenome Pfeifer et al. 86 https://github.com/pievos101/PopGenome Picard ‘‘Picard Toolkit.’’ 2019.

    CRISPR:

    Article Title: Immune gene diversity and STING1 variants in shaping cancer immunity across different genetic ancestry populations.
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Phospho-TBK1/NAK (Ser172) Cell Signaling Technology Cat #5483; RRID: AB_2737027 TBK1/NAK (D1B4) Cell Signaling Technology Cat #3504; RRID: AB_2255663 Phospho-Stat1 (Tyr701) Cell Signaling Technology Cat #7649; RRID: AB_10950970 Stat1 (D1K9Y) Cell Signaling Technology Cat #14994; RRID: AB_2737027 STING (D2P2F) Cell Signaling Technology Cat #13647; RRID: AB_2732796 β-Actin Cell Signaling Technology Cat #4970; RRID: AB_2223172 Anti-mouse IgG Cell Signaling Technology Cat #7076; RRID: AB_330924 Anti-rabbit IgG Cell Signaling Technology Cat #7074; RRID: AB_2099233 Chemicals, peptides, and recombinant proteins RBN2397 Selleckchem Cat# S8993 2’3’-cGAMP Invivogen Cat# tlrl-nacga23-1 poly(dA:dT) Invivogen Cat# tlrl-patn-1 FuGENE 6 Promega Cat# E2691 Lipofectamine 2000 Invitrogen Cat#11668019 MTT Sigma-Aldrich 475989 Crystal violet solution Sigma-Aldrich HT901 Polybrene infection/transfection reagent Sigma-Aldrich TR-1003-G Puromycin Sigma-Aldrich P8833 Hygromycin Roche 10843555001 Blotting-grade blocker Bio-Rad 1706404 Immobilon forte western HRP substrate Millipore Sigma WBLUF0500 CellTracker TM Blue CMF2HC Dye Invitrogen C12881 Critical commercial assays rhAmp® Genotyping Master Mix IDT Cat#1076016 rhAmp® Reporter Mix w/Reference IDT Cat#1076022 STING1 R71H rs11554776 IDT Cat# CD.GT.KJQS7656.1 STING1 G230A rs78233829 IDT Cat# Hs.GT.rs78233829.G.1 STING1 R232H rs1131769 IDT Cat# CD.GT.HPNL6434.1 STING1 R293Q rs7380824 IDT Cat# Hs.GT.rs7380824.T.1 High-Capacity RNA-to-cDNA Kit Applied Biosystems Cat#4368813 PowerUp SYBR Green Applied Biosystems Cat# A25742 Mammalian protein extraction reagent Pierce Cat# 78505 BCA protein assay kit Pierce Cat# 23225 Transwell Permeable Supports Corning Cat# 3422 Human IFN-beta DuoSet ELISA kit R&D Systems Cat# DY814-05 QUANTI-Blue TM Solution Invivogen Cat# rep-qbs QUANTI-Luc TM 4 Lucia/Gaussia Invivogen Cat# rep-qlc4lg1 Deposited data 1000 Genomes Phase 3 data 1000 Genomes project https://www.internationalgenome.org/ data-portal/data-collection/grch38 SNP array data for TCGA TCGA project https://gdc-portal.nci.nih.gov RNAseq data for TCGA UCSC Toil RNAseq Recompute Compendium https://xenabrowser.net/datapages/ ?hub=https://toil.xenahubs.net:443 Segmentation data for TCGA the GDAC Firehose of the Broad Institute http://gdac.broadinstitute.org/ (Continued on next page) 18 Cell Reports 45, 116882, February 24, 2026 .. REAGENT or RESOURCE SOURCE IDENTIFIER Mutation profiles for TCGA TCGA Multi-Center Mutation Calling in Multiple Cancers (MC3) project https://doi.org/10.7303/syn7214402 Fusion profiles for TCGA TumorFusions data portal http://tumorfusions.org/ Genomic profiles for cancer cell lines the Dependency Map (DepMap) portal https://depmap.org/portal/ CRISPR for cancer cell lines the Score projects https://doi.org/10.6084/m9.figshare.c .5289226.v1 TCGA tumors infiltration TIMER2.0 http://timer.cistrome.org RNA sequencing This paper GEO: GSE314074 Data generated in this study This paper http://52.25.87.215/IDmap/ Experimental models: Cell lines 293T ATCC Cat #CRL-3216; RRID: CVCL_0063 CAL27 ATCC Cat #CRL-2095; RRID: CVCL_1107 NK-92 ATCC Cat #CRL-2407; RRID: CVCL_2142 Jurkat ATCC Cat #TIB-152; RRID: CVCL_0065 HCC1143 ATCC Cat #CRL-2321; RRID: CVCL_1245 NCI-H1373 ATCC Cat #CRL-5866; RRID: CVCL_1465 BHY DSMZ Cat #ACC-404; RRID: CVCL_1086 293-Dual TM Null Cells InvivoGen Cat #293d-null TOV-21G ATCC Cat # CRL-3577; RRID: CVCL_3613 SK-OV-3 NCI Development Therapeutics Program RRID: CVCL_0532 HARA JCRB Cat # JCRB1080.0; RRID: CVCL_2914 SNG-M JCRB Cat # JCRB0179; RRID: CVCL_1707 A-253 ATCC Cat# HTB-41 Caov-3 ATCC Cat# HTB-75 Detroit 562 ATCC Cat# CCL-138 DU 145 ATCC Cat# HTB-81 FaDu ATCC Cat# HTB-43 HCC1937 ATCC Cat# CRL-2336 HCC1954 ATCC Cat# CRL-2338 HCC38 ATCC Cat# CRL-2314 Hs 578T ATCC Cat# HTB-126 HT-29 ATCC Cat# HTB-38 LoVo ATCC Cat# CCL-229 MDA-MB-231 ATCC Cat# HTB-26 MDA-MB-361 ATCC Cat# HTB-27 MES-SA ATCC Cat# CRL-1976 OVCAR-8 NCI Development Therapeutics Program RRID:CVCL_1629 PEO1 Sigma Aldrich Cat# 10032308 SCC-9 ATCC Cat# CRL-1629 SCC-15 ATCC Cat# CRL-1623 SCC-25 ATCC Cat# CRL-1628 SK-BR-3 ATCC Cat# HTB-30 SK-CO-1 ATCC Cat# HTB-39 SW 620 ATCC Cat# CCL-227 U-87 MG ATCC Cat# HTB-14 UPCI-SCC-040 DSMZ Cat# ACC-660 UPCI-SCC-074 DSMZ Cat# ACC-664 (Continued on next page) Cell Reports 45, 116882, February 24, 2026 19 .. REAGENT or RESOURCE SOURCE IDENTIFIER UWB1.289 ATCC Cat# CRL-2945 Oligonucleotides 5’ STING1 gRNA forward: 5 ′ -CACCGAGAATCTAGACAGACGGGCG-3 ′ This paper NA 5’ STING1 gRNA reverse: 5 ′ -AAACCGCCCGTCTGTCTAGATTCTC-3 ′ This paper NA 3’ STING1 gRNA forward: 5 ′ -CACCGTGCTCCACACAGACACACAT-3 ′ This paper NA 3’ STING1 gRNA reverse: 5 ′ -AAACATGTGTGTCTGTGTGGAGCAC-3 ′ This paper NA STING1 knockout allele forward: 5 ′ -TGCCATCCTAGCCTCACTCTCCA-3 ′ This paper NA STING1 knockout allele reverse: 5 ′ -AAGGCCCCCACTGACTCTGTCTT-3 ′ This paper NA STING1 WT allele forward: 5 ′ -ACCTGTGGTCTCCCTGGGTC-3 ′ This paper NA STING1 WT allele reverse: 5 ′ -GGCAGGGCTAGGCATCAAGG-3 ′ This paper NA Forward primer for CXCL10: 5 ′ -CCACGTGTTGAGATCATTGC-3 ′ This paper NA Reverse primer for CXCL10: 5 ′ -CCTCTGTGTGGTCCATCCTT-3 ′ This paper NA Forward primer for CCL5: 5 ′ -TACACCAGTGGCAAGTGCTC-3 ′ This paper NA Reverse primer for CCL5: 5 ′ -TACACCAGTGGCAAGTGCTC-3 ′ This paper NA Forward primer for STAT1: 5 ′ -ATGGCAGTCTGGCGGCTGAATT-3 ′ This paper NA Reverse primer for STAT1: 5 ′ -CCAAACCAGGCTGGCACAATTG-3 ′ This paper NA Forward primer for IFNB1: 5 ′ -TGCTCTGGCACAACAGGTAG-3 ′ This paper NA Reverse primer for IFNB1: 5 ′ -CAGGAGAGCAATTTGGAGGA-3 ′ This paper NA Forward primer for GAPDH: 5 ′ -ACACCATGGGGAAGGTGAAG-3 ′ This paper NA Reverse primer for GAPDH: 5 ′ -AAGGGGTCATTGATGGCAAC-3 ′ This paper NA Recombinant DNA LentiCRISPRv2 Sanjana et al. 83 Addgene #52961 pMSCV-hygro-STING Cerboni et al. 84 Addgene #102598 pMSCV-hygro-STING HAQ Cerboni et al. 84 Addgene #102600 pSBbi-RP Hu CXCR3 Unpublished Addgene #183255 Software and algorithms SnpEff Cingolani et al. 85 https://pcingola.github.io/SnpEff/ popgenome Pfeifer et al. 86 https://github.com/pievos101/PopGenome Picard ‘‘Picard Toolkit.’’ 2019.

    RNA Sequencing:

    Article Title: Immune gene diversity and STING1 variants in shaping cancer immunity across different genetic ancestry populations.
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Phospho-TBK1/NAK (Ser172) Cell Signaling Technology Cat #5483; RRID: AB_2737027 TBK1/NAK (D1B4) Cell Signaling Technology Cat #3504; RRID: AB_2255663 Phospho-Stat1 (Tyr701) Cell Signaling Technology Cat #7649; RRID: AB_10950970 Stat1 (D1K9Y) Cell Signaling Technology Cat #14994; RRID: AB_2737027 STING (D2P2F) Cell Signaling Technology Cat #13647; RRID: AB_2732796 β-Actin Cell Signaling Technology Cat #4970; RRID: AB_2223172 Anti-mouse IgG Cell Signaling Technology Cat #7076; RRID: AB_330924 Anti-rabbit IgG Cell Signaling Technology Cat #7074; RRID: AB_2099233 Chemicals, peptides, and recombinant proteins RBN2397 Selleckchem Cat# S8993 2’3’-cGAMP Invivogen Cat# tlrl-nacga23-1 poly(dA:dT) Invivogen Cat# tlrl-patn-1 FuGENE 6 Promega Cat# E2691 Lipofectamine 2000 Invitrogen Cat#11668019 MTT Sigma-Aldrich 475989 Crystal violet solution Sigma-Aldrich HT901 Polybrene infection/transfection reagent Sigma-Aldrich TR-1003-G Puromycin Sigma-Aldrich P8833 Hygromycin Roche 10843555001 Blotting-grade blocker Bio-Rad 1706404 Immobilon forte western HRP substrate Millipore Sigma WBLUF0500 CellTracker TM Blue CMF2HC Dye Invitrogen C12881 Critical commercial assays rhAmp® Genotyping Master Mix IDT Cat#1076016 rhAmp® Reporter Mix w/Reference IDT Cat#1076022 STING1 R71H rs11554776 IDT Cat# CD.GT.KJQS7656.1 STING1 G230A rs78233829 IDT Cat# Hs.GT.rs78233829.G.1 STING1 R232H rs1131769 IDT Cat# CD.GT.HPNL6434.1 STING1 R293Q rs7380824 IDT Cat# Hs.GT.rs7380824.T.1 High-Capacity RNA-to-cDNA Kit Applied Biosystems Cat#4368813 PowerUp SYBR Green Applied Biosystems Cat# A25742 Mammalian protein extraction reagent Pierce Cat# 78505 BCA protein assay kit Pierce Cat# 23225 Transwell Permeable Supports Corning Cat# 3422 Human IFN-beta DuoSet ELISA kit R&D Systems Cat# DY814-05 QUANTI-Blue TM Solution Invivogen Cat# rep-qbs QUANTI-Luc TM 4 Lucia/Gaussia Invivogen Cat# rep-qlc4lg1 Deposited data 1000 Genomes Phase 3 data 1000 Genomes project https://www.internationalgenome.org/ data-portal/data-collection/grch38 SNP array data for TCGA TCGA project https://gdc-portal.nci.nih.gov RNAseq data for TCGA UCSC Toil RNAseq Recompute Compendium https://xenabrowser.net/datapages/ ?hub=https://toil.xenahubs.net:443 Segmentation data for TCGA the GDAC Firehose of the Broad Institute http://gdac.broadinstitute.org/ (Continued on next page) 18 Cell Reports 45, 116882, February 24, 2026 .. REAGENT or RESOURCE SOURCE IDENTIFIER Mutation profiles for TCGA TCGA Multi-Center Mutation Calling in Multiple Cancers (MC3) project https://doi.org/10.7303/syn7214402 Fusion profiles for TCGA TumorFusions data portal http://tumorfusions.org/ Genomic profiles for cancer cell lines the Dependency Map (DepMap) portal https://depmap.org/portal/ CRISPR for cancer cell lines the Score projects https://doi.org/10.6084/m9.figshare.c .5289226.v1 TCGA tumors infiltration TIMER2.0 http://timer.cistrome.org RNA sequencing This paper GEO: GSE314074 Data generated in this study This paper http://52.25.87.215/IDmap/ Experimental models: Cell lines 293T ATCC Cat #CRL-3216; RRID: CVCL_0063 CAL27 ATCC Cat #CRL-2095; RRID: CVCL_1107 NK-92 ATCC Cat #CRL-2407; RRID: CVCL_2142 Jurkat ATCC Cat #TIB-152; RRID: CVCL_0065 HCC1143 ATCC Cat #CRL-2321; RRID: CVCL_1245 NCI-H1373 ATCC Cat #CRL-5866; RRID: CVCL_1465 BHY DSMZ Cat #ACC-404; RRID: CVCL_1086 293-Dual TM Null Cells InvivoGen Cat #293d-null TOV-21G ATCC Cat # CRL-3577; RRID: CVCL_3613 SK-OV-3 NCI Development Therapeutics Program RRID: CVCL_0532 HARA JCRB Cat # JCRB1080.0; RRID: CVCL_2914 SNG-M JCRB Cat # JCRB0179; RRID: CVCL_1707 A-253 ATCC Cat# HTB-41 Caov-3 ATCC Cat# HTB-75 Detroit 562 ATCC Cat# CCL-138 DU 145 ATCC Cat# HTB-81 FaDu ATCC Cat# HTB-43 HCC1937 ATCC Cat# CRL-2336 HCC1954 ATCC Cat# CRL-2338 HCC38 ATCC Cat# CRL-2314 Hs 578T ATCC Cat# HTB-126 HT-29 ATCC Cat# HTB-38 LoVo ATCC Cat# CCL-229 MDA-MB-231 ATCC Cat# HTB-26 MDA-MB-361 ATCC Cat# HTB-27 MES-SA ATCC Cat# CRL-1976 OVCAR-8 NCI Development Therapeutics Program RRID:CVCL_1629 PEO1 Sigma Aldrich Cat# 10032308 SCC-9 ATCC Cat# CRL-1629 SCC-15 ATCC Cat# CRL-1623 SCC-25 ATCC Cat# CRL-1628 SK-BR-3 ATCC Cat# HTB-30 SK-CO-1 ATCC Cat# HTB-39 SW 620 ATCC Cat# CCL-227 U-87 MG ATCC Cat# HTB-14 UPCI-SCC-040 DSMZ Cat# ACC-660 UPCI-SCC-074 DSMZ Cat# ACC-664 (Continued on next page) Cell Reports 45, 116882, February 24, 2026 19 .. REAGENT or RESOURCE SOURCE IDENTIFIER UWB1.289 ATCC Cat# CRL-2945 Oligonucleotides 5’ STING1 gRNA forward: 5 ′ -CACCGAGAATCTAGACAGACGGGCG-3 ′ This paper NA 5’ STING1 gRNA reverse: 5 ′ -AAACCGCCCGTCTGTCTAGATTCTC-3 ′ This paper NA 3’ STING1 gRNA forward: 5 ′ -CACCGTGCTCCACACAGACACACAT-3 ′ This paper NA 3’ STING1 gRNA reverse: 5 ′ -AAACATGTGTGTCTGTGTGGAGCAC-3 ′ This paper NA STING1 knockout allele forward: 5 ′ -TGCCATCCTAGCCTCACTCTCCA-3 ′ This paper NA STING1 knockout allele reverse: 5 ′ -AAGGCCCCCACTGACTCTGTCTT-3 ′ This paper NA STING1 WT allele forward: 5 ′ -ACCTGTGGTCTCCCTGGGTC-3 ′ This paper NA STING1 WT allele reverse: 5 ′ -GGCAGGGCTAGGCATCAAGG-3 ′ This paper NA Forward primer for CXCL10: 5 ′ -CCACGTGTTGAGATCATTGC-3 ′ This paper NA Reverse primer for CXCL10: 5 ′ -CCTCTGTGTGGTCCATCCTT-3 ′ This paper NA Forward primer for CCL5: 5 ′ -TACACCAGTGGCAAGTGCTC-3 ′ This paper NA Reverse primer for CCL5: 5 ′ -TACACCAGTGGCAAGTGCTC-3 ′ This paper NA Forward primer for STAT1: 5 ′ -ATGGCAGTCTGGCGGCTGAATT-3 ′ This paper NA Reverse primer for STAT1: 5 ′ -CCAAACCAGGCTGGCACAATTG-3 ′ This paper NA Forward primer for IFNB1: 5 ′ -TGCTCTGGCACAACAGGTAG-3 ′ This paper NA Reverse primer for IFNB1: 5 ′ -CAGGAGAGCAATTTGGAGGA-3 ′ This paper NA Forward primer for GAPDH: 5 ′ -ACACCATGGGGAAGGTGAAG-3 ′ This paper NA Reverse primer for GAPDH: 5 ′ -AAGGGGTCATTGATGGCAAC-3 ′ This paper NA Recombinant DNA LentiCRISPRv2 Sanjana et al. 83 Addgene #52961 pMSCV-hygro-STING Cerboni et al. 84 Addgene #102598 pMSCV-hygro-STING HAQ Cerboni et al. 84 Addgene #102600 pSBbi-RP Hu CXCR3 Unpublished Addgene #183255 Software and algorithms SnpEff Cingolani et al. 85 https://pcingola.github.io/SnpEff/ popgenome Pfeifer et al. 86 https://github.com/pievos101/PopGenome Picard ‘‘Picard Toolkit.’’ 2019.

    Generated:

    Article Title: Immune gene diversity and STING1 variants in shaping cancer immunity across different genetic ancestry populations.
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Phospho-TBK1/NAK (Ser172) Cell Signaling Technology Cat #5483; RRID: AB_2737027 TBK1/NAK (D1B4) Cell Signaling Technology Cat #3504; RRID: AB_2255663 Phospho-Stat1 (Tyr701) Cell Signaling Technology Cat #7649; RRID: AB_10950970 Stat1 (D1K9Y) Cell Signaling Technology Cat #14994; RRID: AB_2737027 STING (D2P2F) Cell Signaling Technology Cat #13647; RRID: AB_2732796 β-Actin Cell Signaling Technology Cat #4970; RRID: AB_2223172 Anti-mouse IgG Cell Signaling Technology Cat #7076; RRID: AB_330924 Anti-rabbit IgG Cell Signaling Technology Cat #7074; RRID: AB_2099233 Chemicals, peptides, and recombinant proteins RBN2397 Selleckchem Cat# S8993 2’3’-cGAMP Invivogen Cat# tlrl-nacga23-1 poly(dA:dT) Invivogen Cat# tlrl-patn-1 FuGENE 6 Promega Cat# E2691 Lipofectamine 2000 Invitrogen Cat#11668019 MTT Sigma-Aldrich 475989 Crystal violet solution Sigma-Aldrich HT901 Polybrene infection/transfection reagent Sigma-Aldrich TR-1003-G Puromycin Sigma-Aldrich P8833 Hygromycin Roche 10843555001 Blotting-grade blocker Bio-Rad 1706404 Immobilon forte western HRP substrate Millipore Sigma WBLUF0500 CellTracker TM Blue CMF2HC Dye Invitrogen C12881 Critical commercial assays rhAmp® Genotyping Master Mix IDT Cat#1076016 rhAmp® Reporter Mix w/Reference IDT Cat#1076022 STING1 R71H rs11554776 IDT Cat# CD.GT.KJQS7656.1 STING1 G230A rs78233829 IDT Cat# Hs.GT.rs78233829.G.1 STING1 R232H rs1131769 IDT Cat# CD.GT.HPNL6434.1 STING1 R293Q rs7380824 IDT Cat# Hs.GT.rs7380824.T.1 High-Capacity RNA-to-cDNA Kit Applied Biosystems Cat#4368813 PowerUp SYBR Green Applied Biosystems Cat# A25742 Mammalian protein extraction reagent Pierce Cat# 78505 BCA protein assay kit Pierce Cat# 23225 Transwell Permeable Supports Corning Cat# 3422 Human IFN-beta DuoSet ELISA kit R&D Systems Cat# DY814-05 QUANTI-Blue TM Solution Invivogen Cat# rep-qbs QUANTI-Luc TM 4 Lucia/Gaussia Invivogen Cat# rep-qlc4lg1 Deposited data 1000 Genomes Phase 3 data 1000 Genomes project https://www.internationalgenome.org/ data-portal/data-collection/grch38 SNP array data for TCGA TCGA project https://gdc-portal.nci.nih.gov RNAseq data for TCGA UCSC Toil RNAseq Recompute Compendium https://xenabrowser.net/datapages/ ?hub=https://toil.xenahubs.net:443 Segmentation data for TCGA the GDAC Firehose of the Broad Institute http://gdac.broadinstitute.org/ (Continued on next page) 18 Cell Reports 45, 116882, February 24, 2026 .. REAGENT or RESOURCE SOURCE IDENTIFIER Mutation profiles for TCGA TCGA Multi-Center Mutation Calling in Multiple Cancers (MC3) project https://doi.org/10.7303/syn7214402 Fusion profiles for TCGA TumorFusions data portal http://tumorfusions.org/ Genomic profiles for cancer cell lines the Dependency Map (DepMap) portal https://depmap.org/portal/ CRISPR for cancer cell lines the Score projects https://doi.org/10.6084/m9.figshare.c .5289226.v1 TCGA tumors infiltration TIMER2.0 http://timer.cistrome.org RNA sequencing This paper GEO: GSE314074 Data generated in this study This paper http://52.25.87.215/IDmap/ Experimental models: Cell lines 293T ATCC Cat #CRL-3216; RRID: CVCL_0063 CAL27 ATCC Cat #CRL-2095; RRID: CVCL_1107 NK-92 ATCC Cat #CRL-2407; RRID: CVCL_2142 Jurkat ATCC Cat #TIB-152; RRID: CVCL_0065 HCC1143 ATCC Cat #CRL-2321; RRID: CVCL_1245 NCI-H1373 ATCC Cat #CRL-5866; RRID: CVCL_1465 BHY DSMZ Cat #ACC-404; RRID: CVCL_1086 293-Dual TM Null Cells InvivoGen Cat #293d-null TOV-21G ATCC Cat # CRL-3577; RRID: CVCL_3613 SK-OV-3 NCI Development Therapeutics Program RRID: CVCL_0532 HARA JCRB Cat # JCRB1080.0; RRID: CVCL_2914 SNG-M JCRB Cat # JCRB0179; RRID: CVCL_1707 A-253 ATCC Cat# HTB-41 Caov-3 ATCC Cat# HTB-75 Detroit 562 ATCC Cat# CCL-138 DU 145 ATCC Cat# HTB-81 FaDu ATCC Cat# HTB-43 HCC1937 ATCC Cat# CRL-2336 HCC1954 ATCC Cat# CRL-2338 HCC38 ATCC Cat# CRL-2314 Hs 578T ATCC Cat# HTB-126 HT-29 ATCC Cat# HTB-38 LoVo ATCC Cat# CCL-229 MDA-MB-231 ATCC Cat# HTB-26 MDA-MB-361 ATCC Cat# HTB-27 MES-SA ATCC Cat# CRL-1976 OVCAR-8 NCI Development Therapeutics Program RRID:CVCL_1629 PEO1 Sigma Aldrich Cat# 10032308 SCC-9 ATCC Cat# CRL-1629 SCC-15 ATCC Cat# CRL-1623 SCC-25 ATCC Cat# CRL-1628 SK-BR-3 ATCC Cat# HTB-30 SK-CO-1 ATCC Cat# HTB-39 SW 620 ATCC Cat# CCL-227 U-87 MG ATCC Cat# HTB-14 UPCI-SCC-040 DSMZ Cat# ACC-660 UPCI-SCC-074 DSMZ Cat# ACC-664 (Continued on next page) Cell Reports 45, 116882, February 24, 2026 19 .. REAGENT or RESOURCE SOURCE IDENTIFIER UWB1.289 ATCC Cat# CRL-2945 Oligonucleotides 5’ STING1 gRNA forward: 5 ′ -CACCGAGAATCTAGACAGACGGGCG-3 ′ This paper NA 5’ STING1 gRNA reverse: 5 ′ -AAACCGCCCGTCTGTCTAGATTCTC-3 ′ This paper NA 3’ STING1 gRNA forward: 5 ′ -CACCGTGCTCCACACAGACACACAT-3 ′ This paper NA 3’ STING1 gRNA reverse: 5 ′ -AAACATGTGTGTCTGTGTGGAGCAC-3 ′ This paper NA STING1 knockout allele forward: 5 ′ -TGCCATCCTAGCCTCACTCTCCA-3 ′ This paper NA STING1 knockout allele reverse: 5 ′ -AAGGCCCCCACTGACTCTGTCTT-3 ′ This paper NA STING1 WT allele forward: 5 ′ -ACCTGTGGTCTCCCTGGGTC-3 ′ This paper NA STING1 WT allele reverse: 5 ′ -GGCAGGGCTAGGCATCAAGG-3 ′ This paper NA Forward primer for CXCL10: 5 ′ -CCACGTGTTGAGATCATTGC-3 ′ This paper NA Reverse primer for CXCL10: 5 ′ -CCTCTGTGTGGTCCATCCTT-3 ′ This paper NA Forward primer for CCL5: 5 ′ -TACACCAGTGGCAAGTGCTC-3 ′ This paper NA Reverse primer for CCL5: 5 ′ -TACACCAGTGGCAAGTGCTC-3 ′ This paper NA Forward primer for STAT1: 5 ′ -ATGGCAGTCTGGCGGCTGAATT-3 ′ This paper NA Reverse primer for STAT1: 5 ′ -CCAAACCAGGCTGGCACAATTG-3 ′ This paper NA Forward primer for IFNB1: 5 ′ -TGCTCTGGCACAACAGGTAG-3 ′ This paper NA Reverse primer for IFNB1: 5 ′ -CAGGAGAGCAATTTGGAGGA-3 ′ This paper NA Forward primer for GAPDH: 5 ′ -ACACCATGGGGAAGGTGAAG-3 ′ This paper NA Reverse primer for GAPDH: 5 ′ -AAGGGGTCATTGATGGCAAC-3 ′ This paper NA Recombinant DNA LentiCRISPRv2 Sanjana et al. 83 Addgene #52961 pMSCV-hygro-STING Cerboni et al. 84 Addgene #102598 pMSCV-hygro-STING HAQ Cerboni et al. 84 Addgene #102600 pSBbi-RP Hu CXCR3 Unpublished Addgene #183255 Software and algorithms SnpEff Cingolani et al. 85 https://pcingola.github.io/SnpEff/ popgenome Pfeifer et al. 86 https://github.com/pievos101/PopGenome Picard ‘‘Picard Toolkit.’’ 2019.

    Multiple Displacement Amplification:

    Article Title: Immune gene diversity and STING1 variants in shaping cancer immunity across different genetic ancestry populations.
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Phospho-TBK1/NAK (Ser172) Cell Signaling Technology Cat #5483; RRID: AB_2737027 TBK1/NAK (D1B4) Cell Signaling Technology Cat #3504; RRID: AB_2255663 Phospho-Stat1 (Tyr701) Cell Signaling Technology Cat #7649; RRID: AB_10950970 Stat1 (D1K9Y) Cell Signaling Technology Cat #14994; RRID: AB_2737027 STING (D2P2F) Cell Signaling Technology Cat #13647; RRID: AB_2732796 β-Actin Cell Signaling Technology Cat #4970; RRID: AB_2223172 Anti-mouse IgG Cell Signaling Technology Cat #7076; RRID: AB_330924 Anti-rabbit IgG Cell Signaling Technology Cat #7074; RRID: AB_2099233 Chemicals, peptides, and recombinant proteins RBN2397 Selleckchem Cat# S8993 2’3’-cGAMP Invivogen Cat# tlrl-nacga23-1 poly(dA:dT) Invivogen Cat# tlrl-patn-1 FuGENE 6 Promega Cat# E2691 Lipofectamine 2000 Invitrogen Cat#11668019 MTT Sigma-Aldrich 475989 Crystal violet solution Sigma-Aldrich HT901 Polybrene infection/transfection reagent Sigma-Aldrich TR-1003-G Puromycin Sigma-Aldrich P8833 Hygromycin Roche 10843555001 Blotting-grade blocker Bio-Rad 1706404 Immobilon forte western HRP substrate Millipore Sigma WBLUF0500 CellTracker TM Blue CMF2HC Dye Invitrogen C12881 Critical commercial assays rhAmp® Genotyping Master Mix IDT Cat#1076016 rhAmp® Reporter Mix w/Reference IDT Cat#1076022 STING1 R71H rs11554776 IDT Cat# CD.GT.KJQS7656.1 STING1 G230A rs78233829 IDT Cat# Hs.GT.rs78233829.G.1 STING1 R232H rs1131769 IDT Cat# CD.GT.HPNL6434.1 STING1 R293Q rs7380824 IDT Cat# Hs.GT.rs7380824.T.1 High-Capacity RNA-to-cDNA Kit Applied Biosystems Cat#4368813 PowerUp SYBR Green Applied Biosystems Cat# A25742 Mammalian protein extraction reagent Pierce Cat# 78505 BCA protein assay kit Pierce Cat# 23225 Transwell Permeable Supports Corning Cat# 3422 Human IFN-beta DuoSet ELISA kit R&D Systems Cat# DY814-05 QUANTI-Blue TM Solution Invivogen Cat# rep-qbs QUANTI-Luc TM 4 Lucia/Gaussia Invivogen Cat# rep-qlc4lg1 Deposited data 1000 Genomes Phase 3 data 1000 Genomes project https://www.internationalgenome.org/ data-portal/data-collection/grch38 SNP array data for TCGA TCGA project https://gdc-portal.nci.nih.gov RNAseq data for TCGA UCSC Toil RNAseq Recompute Compendium https://xenabrowser.net/datapages/ ?hub=https://toil.xenahubs.net:443 Segmentation data for TCGA the GDAC Firehose of the Broad Institute http://gdac.broadinstitute.org/ (Continued on next page) 18 Cell Reports 45, 116882, February 24, 2026 .. REAGENT or RESOURCE SOURCE IDENTIFIER Mutation profiles for TCGA TCGA Multi-Center Mutation Calling in Multiple Cancers (MC3) project https://doi.org/10.7303/syn7214402 Fusion profiles for TCGA TumorFusions data portal http://tumorfusions.org/ Genomic profiles for cancer cell lines the Dependency Map (DepMap) portal https://depmap.org/portal/ CRISPR for cancer cell lines the Score projects https://doi.org/10.6084/m9.figshare.c .5289226.v1 TCGA tumors infiltration TIMER2.0 http://timer.cistrome.org RNA sequencing This paper GEO: GSE314074 Data generated in this study This paper http://52.25.87.215/IDmap/ Experimental models: Cell lines 293T ATCC Cat #CRL-3216; RRID: CVCL_0063 CAL27 ATCC Cat #CRL-2095; RRID: CVCL_1107 NK-92 ATCC Cat #CRL-2407; RRID: CVCL_2142 Jurkat ATCC Cat #TIB-152; RRID: CVCL_0065 HCC1143 ATCC Cat #CRL-2321; RRID: CVCL_1245 NCI-H1373 ATCC Cat #CRL-5866; RRID: CVCL_1465 BHY DSMZ Cat #ACC-404; RRID: CVCL_1086 293-Dual TM Null Cells InvivoGen Cat #293d-null TOV-21G ATCC Cat # CRL-3577; RRID: CVCL_3613 SK-OV-3 NCI Development Therapeutics Program RRID: CVCL_0532 HARA JCRB Cat # JCRB1080.0; RRID: CVCL_2914 SNG-M JCRB Cat # JCRB0179; RRID: CVCL_1707 A-253 ATCC Cat# HTB-41 Caov-3 ATCC Cat# HTB-75 Detroit 562 ATCC Cat# CCL-138 DU 145 ATCC Cat# HTB-81 FaDu ATCC Cat# HTB-43 HCC1937 ATCC Cat# CRL-2336 HCC1954 ATCC Cat# CRL-2338 HCC38 ATCC Cat# CRL-2314 Hs 578T ATCC Cat# HTB-126 HT-29 ATCC Cat# HTB-38 LoVo ATCC Cat# CCL-229 MDA-MB-231 ATCC Cat# HTB-26 MDA-MB-361 ATCC Cat# HTB-27 MES-SA ATCC Cat# CRL-1976 OVCAR-8 NCI Development Therapeutics Program RRID:CVCL_1629 PEO1 Sigma Aldrich Cat# 10032308 SCC-9 ATCC Cat# CRL-1629 SCC-15 ATCC Cat# CRL-1623 SCC-25 ATCC Cat# CRL-1628 SK-BR-3 ATCC Cat# HTB-30 SK-CO-1 ATCC Cat# HTB-39 SW 620 ATCC Cat# CCL-227 U-87 MG ATCC Cat# HTB-14 UPCI-SCC-040 DSMZ Cat# ACC-660 UPCI-SCC-074 DSMZ Cat# ACC-664 (Continued on next page) Cell Reports 45, 116882, February 24, 2026 19 .. REAGENT or RESOURCE SOURCE IDENTIFIER UWB1.289 ATCC Cat# CRL-2945 Oligonucleotides 5’ STING1 gRNA forward: 5 ′ -CACCGAGAATCTAGACAGACGGGCG-3 ′ This paper NA 5’ STING1 gRNA reverse: 5 ′ -AAACCGCCCGTCTGTCTAGATTCTC-3 ′ This paper NA 3’ STING1 gRNA forward: 5 ′ -CACCGTGCTCCACACAGACACACAT-3 ′ This paper NA 3’ STING1 gRNA reverse: 5 ′ -AAACATGTGTGTCTGTGTGGAGCAC-3 ′ This paper NA STING1 knockout allele forward: 5 ′ -TGCCATCCTAGCCTCACTCTCCA-3 ′ This paper NA STING1 knockout allele reverse: 5 ′ -AAGGCCCCCACTGACTCTGTCTT-3 ′ This paper NA STING1 WT allele forward: 5 ′ -ACCTGTGGTCTCCCTGGGTC-3 ′ This paper NA STING1 WT allele reverse: 5 ′ -GGCAGGGCTAGGCATCAAGG-3 ′ This paper NA Forward primer for CXCL10: 5 ′ -CCACGTGTTGAGATCATTGC-3 ′ This paper NA Reverse primer for CXCL10: 5 ′ -CCTCTGTGTGGTCCATCCTT-3 ′ This paper NA Forward primer for CCL5: 5 ′ -TACACCAGTGGCAAGTGCTC-3 ′ This paper NA Reverse primer for CCL5: 5 ′ -TACACCAGTGGCAAGTGCTC-3 ′ This paper NA Forward primer for STAT1: 5 ′ -ATGGCAGTCTGGCGGCTGAATT-3 ′ This paper NA Reverse primer for STAT1: 5 ′ -CCAAACCAGGCTGGCACAATTG-3 ′ This paper NA Forward primer for IFNB1: 5 ′ -TGCTCTGGCACAACAGGTAG-3 ′ This paper NA Reverse primer for IFNB1: 5 ′ -CAGGAGAGCAATTTGGAGGA-3 ′ This paper NA Forward primer for GAPDH: 5 ′ -ACACCATGGGGAAGGTGAAG-3 ′ This paper NA Reverse primer for GAPDH: 5 ′ -AAGGGGTCATTGATGGCAAC-3 ′ This paper NA Recombinant DNA LentiCRISPRv2 Sanjana et al. 83 Addgene #52961 pMSCV-hygro-STING Cerboni et al. 84 Addgene #102598 pMSCV-hygro-STING HAQ Cerboni et al. 84 Addgene #102600 pSBbi-RP Hu CXCR3 Unpublished Addgene #183255 Software and algorithms SnpEff Cingolani et al. 85 https://pcingola.github.io/SnpEff/ popgenome Pfeifer et al. 86 https://github.com/pievos101/PopGenome Picard ‘‘Picard Toolkit.’’ 2019.



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